xingxu huang (Addgene inc)
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xingxu huang
Xingxu Huang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xingxu+huang/pGL3-U6-sgRNA-PGK-puromycin+(Plasmid+%2351133)/pmc12884776-268-37-41
Average 94 stars, based on 168 article reviews
Xingxu Huang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xingxu+huang/pGL3-U6-sgRNA-PGK-puromycin+(Plasmid+%2351133)/pmc12884776-268-37-41
Average 94 stars, based on 168 article reviews
xingxu huang - by Bioz Stars,
2026-11
94/100 stars
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Plasmid Preparation:Article Title: Generating a New sgRNA Vector, pGL3-U6-sgRNA-PGK-mRFP-T2A-PuroR, to Improve Base Editing Article Snippet: Unless otherwise specified, DNA primers ( ) were designed using ApE (A plasmid Editor; by M. Wayne Davis) and custom made (Sigma). .. MCS1-EF1α-RFP-T2A-Puro-pA-MCS2 was purchased from System Biosciences (# HR110PA-1). pGL3-U6-sgRNA-PGK-puromycin was a gift from Article Title: PIBF1 regulates trophoblast syncytialization and promotes cardiovascular development Article Snippet: To generate single guide RNAs (sgRNAs) used for floxed PIBF1 mice, the following oligomers were purchased from Macrogen, Inc.: 5′−taggGCTATTTCAGTTTCTCTAAG−3′ and 5′−aaacCTTAGAGAAACTGAAATAGC−3′ for Pibf1 intron 1-specific sgRNA; 5′−taggACTCTATTTCTGCTTCCCGA−3′ and 5′−aaacTCGGGAAGCAGAAATAGAGT−3′ for Pibf1 intron 17-specific sgRNA. .. Each pair of oligomers was annealed and cloned into pUC57-sgRNA vector, a gift from Article Title: m 5 C methylated lncRncr3 –MeCP2 interaction restricts miR124a- initiated neurogenesis Article Snippet: The beads were pelleted by magnet and the supernatant subjected to protein size-separation in 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels (Bio-Rad) and used for western blot experiments. .. To express appropriate sgRNAs in NE-4C cells for deletion of Rncr3 exons 2/3 or for Rncr3 full length KO, the pGL3 vector (Supplementary Table ) with the U6 promoter was used and co-transfected with pCAS9 into cells. pGL3-U6-sgRNA-PGK-puromycin was a gift from Article Title: Effectiveness of Multivitamins vs Folic Acid on Prevention of Neural Tube Defects in Mouse Genetic Models and Human Organoids Article Snippet: .. To express appropriate sgRNAs in human iPS cells for deletion of the HAT domain of hGCN5 , the pGL3 vector with the U6 promoter was used and co‐transfected with pCAS9 into cells. pGL3‐U6‐sgRNA‐PGK‐puromycin was a gift from Article Title: Plxnd1-mediated mechanosensing of blood flow controls the caliber of the Dorsal Aorta via the transcription factor Klf2 Article Snippet: Michel Bagnat ( TgKI(tjp1a-eGFP) pd1252 )[ ], the Zebrafish International Resource Center/ZIRC ( gata1a m651 , klf2b sa43252 , and sih tc300b ). .. Vectors : Chi-Bin Chien and Koichi Kawakami (pCS2FA-transposase, pDestTol2CG2, and p3E-polyA,)[ ], Nathan Lawson (p5E-fliep)[ ], Salim Abdelilah-Seyfried (pME-klf2a)[ ], and Article Title: Comparison of ICSI, IVF, and in vivo derived embryos to produce CRISPR-Cas9 gene-edited pigs for xenotransplantation. Article Snippet: Genome editing in pigs for xenotransplantation has seen significant advances in recent years.. This study compared three methodologies to generate gene-edited embryos, including co-injection of sperm together with the CRISPR-Cas9 system into oocytes, named ICSI-MGE (mediated gene editing); microinjection of CRISPR-Cas9 components into oocytes followed by in vitro fertilization (IVF), and microinjection of in vivo fertilized zygotes with the CRISPR-Cas9 system.. Our goal was to knock-out (KO) porcine genes involved in the biosynthesis of xenoantigens responsible for the hyperacute rejection of interspecific xenografts, namely GGTA1, CMAH, and β4GalNT2. Clone Assay:Article Title: PIBF1 regulates trophoblast syncytialization and promotes cardiovascular development Article Snippet: To generate single guide RNAs (sgRNAs) used for floxed PIBF1 mice, the following oligomers were purchased from Macrogen, Inc.: 5′−taggGCTATTTCAGTTTCTCTAAG−3′ and 5′−aaacCTTAGAGAAACTGAAATAGC−3′ for Pibf1 intron 1-specific sgRNA; 5′−taggACTCTATTTCTGCTTCCCGA−3′ and 5′−aaacTCGGGAAGCAGAAATAGAGT−3′ for Pibf1 intron 17-specific sgRNA. .. Each pair of oligomers was annealed and cloned into pUC57-sgRNA vector, a gift from Article Title: Comparison of ICSI, IVF, and in vivo derived embryos to produce CRISPR-Cas9 gene-edited pigs for xenotransplantation. Article Snippet: Genome editing in pigs for xenotransplantation has seen significant advances in recent years.. This study compared three methodologies to generate gene-edited embryos, including co-injection of sperm together with the CRISPR-Cas9 system into oocytes, named ICSI-MGE (mediated gene editing); microinjection of CRISPR-Cas9 components into oocytes followed by in vitro fertilization (IVF), and microinjection of in vivo fertilized zygotes with the CRISPR-Cas9 system.. Our goal was to knock-out (KO) porcine genes involved in the biosynthesis of xenoantigens responsible for the hyperacute rejection of interspecific xenografts, namely GGTA1, CMAH, and β4GalNT2. In Vitro:Article Title: PIBF1 regulates trophoblast syncytialization and promotes cardiovascular development Article Snippet: To generate single guide RNAs (sgRNAs) used for floxed PIBF1 mice, the following oligomers were purchased from Macrogen, Inc.: 5′−taggGCTATTTCAGTTTCTCTAAG−3′ and 5′−aaacCTTAGAGAAACTGAAATAGC−3′ for Pibf1 intron 1-specific sgRNA; 5′−taggACTCTATTTCTGCTTCCCGA−3′ and 5′−aaacTCGGGAAGCAGAAATAGAGT−3′ for Pibf1 intron 17-specific sgRNA. .. Each pair of oligomers was annealed and cloned into pUC57-sgRNA vector, a gift from other:Article Title: PIBF1 regulates trophoblast syncytialization and promotes cardiovascular development. Article Snippet: After 48 h, the cultured cells were stained with Hoechst 33342 (H1399, Invitrogen) and 2μM Di-8-ANEPPS (D3167, Invitrogen) for 30min, and subsequently imaged by Zeiss LSM 880 Fast Airyscan Confocal system (Carl Zeiss, Jena, Germany). Article Title: RepFluo, a Fast Fluorescent In Vitro Assay of Cas9 Activity Exploiting Melting Curve Analysis Article Snippet: Immediately before use, the protein was diluted in PBS (Merk, Sigma-Aldrich). HAT Assay:Article Title: Effectiveness of Multivitamins vs Folic Acid on Prevention of Neural Tube Defects in Mouse Genetic Models and Human Organoids Article Snippet: .. To express appropriate sgRNAs in human iPS cells for deletion of the HAT domain of hGCN5 , the pGL3 vector with the U6 promoter was used and co‐transfected with pCAS9 into cells. pGL3‐U6‐sgRNA‐PGK‐puromycin was a gift from Expressing:Article Title: Comparison of ICSI, IVF, and in vivo derived embryos to produce CRISPR-Cas9 gene-edited pigs for xenotransplantation. Article Snippet: Genome editing in pigs for xenotransplantation has seen significant advances in recent years.. This study compared three methodologies to generate gene-edited embryos, including co-injection of sperm together with the CRISPR-Cas9 system into oocytes, named ICSI-MGE (mediated gene editing); microinjection of CRISPR-Cas9 components into oocytes followed by in vitro fertilization (IVF), and microinjection of in vivo fertilized zygotes with the CRISPR-Cas9 system.. Our goal was to knock-out (KO) porcine genes involved in the biosynthesis of xenoantigens responsible for the hyperacute rejection of interspecific xenografts, namely GGTA1, CMAH, and β4GalNT2. |